Cat. No.
MABL-3876
Application
deplete, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
huA33
From
Recombinant Antibody
Specificity
hA33 binds specifically to the ectodomain of A33 cell-surface differentation antigen. The antigen is a single-pass type-1 membrane glyoprotein of the immunoglobulin superfamily and, while not present in most normal and tumor cell type, it is expressed on the vast majority (95%) of both primary and metastatic colon cancer cells. The protein is involved in cell-cell recognition and signaling.
Alternative Names
hA33; A33; GPA33; glycoprotein A33; cell surface A33 antigen
UniProt
Q99795
Immunogen
AF5 was prepared by immunizing female BALB/c mice with human amniotic fluid containing AFP, from a pregnancy affected by a neural tube defect, diluted and emulsified in Freund's complete adjuvant.
Application Notes
Antibody First Published
Hemminki et al. Introduction of lysine residues on the light chain constant domain improves the labelling properties of a recombinant Fab fragment. Protein Eng. 1995 Feb;8(2):185-91. PMID:7543205
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
