Cat. No.
MABL-3879
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
G3-F11
From
Recombinant Antibody
Specificity
This antibody recognizes and binds human aspartyl/asparaginyl beta-hydroxylase. The human aspartyl (asparaginyl) beta-hydroxylase (HAAH) is an a-ketoglutarate-dependent dioxygenase, which is a membrane-associated and highly conserved enzyme that hydroxylates epidermal growth factor-like domains in transformation-associated proteins. Overexpression of HAAH is recognized as an indicator for carcinomas in humans.
Alternative Names
HAAH; ASPH; BAH; ASP beta-hydroxylase; Aspartyl beta-hydroxylase; Aspartyl/asparaginyl beta-hydroxylase; Peptide-aspartate beta-dioxygenase; G3/F11; G3; F11
UniProt
Q12797
Immunogen
This antibody was raised against a synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein.
Application Notes
Bcl-2 has been implicated in various cancers, such as lymphoma, melanoma or colorectal cancer. Therefore, multiple immunohistochemical stainings against bcl-2 protein were performed and this antibody was found to be useful in characterisation of molecular features of certain cancers. ICH: One group used bcl-2/100 antibody to stain paraffin-embedded colorectal cancer samples and strong signal, mainly from the colonic cells, was observed in the tissue (Nicholson et al., 2014). Another group noted bcl-2 immunohistochemical staining in both morular and nonmorular papillary thyroid carcinoma samples while trying to identify cliinicopathological features of this carcinoma (Jung et al., 2009). Finally, yet another found that neoplastic cells of the primary sclerosing epithelioid fibrosarcoma (SEF) of the kidney are characterised by a strong anti-bcl2 immunohistochemical staining with this antibody (Ertoy Baydar et al., 2015). WB: Additionally, multiple groups were successfull while using bcl-2/100 antibody to detect bcl-2 via Western blot; for example in HEC cells while investigating the influence of simvastatin combined with kallistatin on paediatric burn patients with sepsis (Dai and Zhao, 2018).
Antibody First Published
Pezzella et al. Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol. 1990 Aug;137(2):225-32. PMID:2201196
Note on publication
This article describes generation and characterisation of the bcl-2/100 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
