Cat. No.
MABL-3416
Application
FACS, WB, ELISA, FC Published Species Reactivity
Isotype
Engineer antibody
Species Reactivity
Species independant
Clone No.
3D5
From
Recombinant Antibody
Specificity
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Alternative Names
polyhistidine tag
UniProt
Immunogen
Purified his tagged Fab fragment.
Application Notes
This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997; PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015; PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A- transfected HEK293 A cells (Akiyama et al., 2019; PMID: 30885301).
Antibody First Published
Lindner P, Bauer K, Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R, Plückthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan;22(1):140-9. PMID:8994661
Note on publication
Describes the making of the antibody, shows it recognised carboxy-terminal oligo- histidine tags (His tags) of a wide variety of proteins in immuno-assays of cell lines with His tag amplifications.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
