Cat. No.
MABL-3420
Application
crystallography, IP, ITC, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
DNA-1
From
Recombinant Antibody
Specificity
This antibody is specific for dT5, with high specificity to sequences greater than 5 nucleotides. It primarily recognizes the thymine DNA base, but it also binds to oligo(dT)15 nucleotides or greater and oligo(dT) of length three to five nucleotides. The antibody also recognizes the abbreviated hairpin sequence LIG5-14.
Alternative Names
dT5; Oligo(dT); Oligo (dT); poly(dT); poly (dT)
UniProt
Immunogen
The original format of this antibody was isolated from a phage display derived from MRL/lpr mice (autoimmune mice). The library was panned against single-stranded calf thymus DNA.
Application Notes
The original format of this antibody (Fab) was generated and characterized. It was shown to bind DNA in an IP assay. and its binding affinity was determined to be (4.4±2.4) × 10-8 M (Calcutt et al., 1993; PMID: 7506692). Fluorescence titration of the antibody with dT5 was performed, and a Kd of ~27 µM was measured (Komissarov et al., 1996; PMID: 8647821). The crystal structure of the Fab fragment in complex with dT5 was determined (Tanner et al., 2001; PMID: 11733999). Isothermal titration calorimetry experiments were used to measure the apparent affinity of the antibody for dT5. The apparent association constant was KA = 6.0 × 104 M-1. Furthermore, the binding of dT5 to the antibody was exothermic, with an apparent ΔHcal under solution conditions of -16.8 kcal/mol (Schuermann et al., 2004; PMID: 15340914). The crystal structure of the Fab version of this antibody was characterized in complex with a DNA ligand corresponding to nucleotides 5–14 of LIG1-17 (LIG5-14) (Ou et al., 2007; PMID: 18028946).
Antibody First Published
Calcutt et al. Isolation and characterization of nucleic acid-binding antibody fragments from autoimmune mice-derived bacteriophage display libraries Gene. 1993 Dec 27;137(1):77-83. PMID:7506692
Note on publication
The original publication explores the isolation and characterization of nucleic acid- binding antibody fragments from autoimmune mice-derived bacteriophage display libraries.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
