Cat. No.
MABL-3431
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
EE tag
Clone No.
3D5/EE_48.K
From
Recombinant Antibody
Specificity
This antibody recognizes and binds a six amino acid long sequence (EYMPME).
Alternative Names
EE epitope tag (EYMPME); Glu-Glu epitope tag
UniProt
Immunogen
This scFv antibody was generated by previously crystallized hexa-histidine-specific 3D5 scFv antibody. The 3D5 scFv was modified in the complementary determining region by random mutagenesis, in conjunction with phage display, to yield scFvs with new biochemical characteristics and binding specificity.
Application Notes
This antibody successfully identifies protein with either internal or C-terminal EE peptides in western blot assays. When expressed in soluble form they recognize the EE peptides in ELISA (PMID:21217145; PMID:24615866).
Antibody First Published
Pai et al. Conversion of scFv peptide-binding specificity for crystal chaperone development. Protein Eng Des Sel. (2011); 24(5): 419–428. PMID:21217145
Note on publication
Describes the generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
