Cat. No.
MABL-3458
Application
Blocking, functional assays, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RMT2-14
From
Recombinant Antibody
Specificity
This antibody is specific for murine TIM-2, a transmembrane protein that is expressed on activated T cells and preferentially on differentiated Th2 cells. A blockade of TIM-2/TIM-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. TIM-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.
Alternative Names
TIMD2; T Cell Immunoglobulin Mucin-2; T Cell immunoglobulin and mucin domain protein 2; TIM3
UniProt
Q8R183
Immunogen
This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.
Application Notes
This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011).
Antibody First Published
Nakae et al. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells. Blood. 2007 Oct 1; 110(7): 2565–2568. PMID:17620455
Note on publication
Describe the original generation of this clone RMT2-1, together with another clone (RMT2-14), of this anti-mouse TIM-2 antibody and their subsequent use for evaluation of the expression of TIM family members on mouse mast cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
