Cat. No.
MABL-3464
Application
Blocking, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
13F3
From
Recombinant Antibody
Specificity
This antibody is specific for murine VISTA.
Alternative Names
PD-1H; V-domain Ig suppressor of T cell activation; V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; V-set domain- containing immunoregulatory receptor; V-set immunoregulatory receptor; Vsir; Dies1
UniProt
Q9D659
Immunogen
This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig fusion protein.
Application Notes
The specificity of this antibody has been confirmed in both ELISA and flow cytometry (Wang et al, 2011). This antibody has been used in flow cytometric analysis to assess VISTA expression in murine lupus models (Ceeraz et al, 2017; Sergent et al, 2018) and mice infected with HSV-1 (Srivastava et al, 2018). This antibody has been shown to block VISTA, and enhances T cell proliferation in vitro (Wang et al, 2011). In mice models, this antibody exacerbates experimental autoimmune encephalomyelitis (Wang et al, 2011) and murine arthritis and lupus disease progression (Sergent et al, 2018). The use of this antibody to achieve a VISTA blockade enhances anti-tumour immune responses in mice models of both transplantable and inducible melanoma, suppressing tumour growth (LeMercier et al, 2014).
Antibody First Published
Wang et al. VISTA, a novel mouse Ig superfamily ligand that negatively regulates T cell responses. J Exp Med. 2011 Mar 14;208(3):577-92. PMID:21383057
Note on publication
Describes the original generation of this antibody, and its exacerbation of EAE disease progression.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
