Cat. No.
MABL-3495
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
CL10
From
Recombinant Antibody
Specificity
Alternative Names
FRβ; Folate receptor 2; Folate-binding protein 2.
UniProt
Q05685
Immunogen
FRβ-transfected RBL2H3 cells.
Application Notes
The antibody binds specifically to FRβ, a member of the folate receptor family. The receptor binds with high affinity to folic acid and folic acid derivatives, and transports 5- methyltetrahydrofolate into the cell. The receptor is expressed on the surface of tumour associated macrophages (TAMs) that promote growth and metastasis of tumours such as gliomas and colon, breast and lung carcinomas. Research has shown FRβ is only expressed on activated macrophages, and thereby a monoclonal antibody that targets the receptor can be useful in the diagnosis of the cancer. Furthermore, the antibody may be conjugated with a toxin, delivering the drug only to TAMs and not to healthy macrophages.
Antibody First Published
Nagai T, Tanaka M, Tsuneyoshi Y, Xu B, Michie SA, Hasui K, Hirano H, Arita K, Matsuyama T. Targeting tumor-associated macrophages in an experimental glioma model with a recombinant immunotoxin to folate receptor beta. Cancer Immunol Immunother. 2009 Oct;58(10):1577-86. PMID:19238383
Note on publication
Describes the expression of folate receptor beta on macrophages in glioblastomas, and the production of an antibody against the receptor which can reduce tumour growth.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
