Cat. No.
MABL-3559
Application
grating-coupledinterferometry, inhibition, therapeutics, ELISA
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus2 (SARS-Cov-2)
Clone No.
Sb#68
From
Recombinant Antibody
Specificity
This antibody recognizes and binds the SARS CoV 2 C-terminal receptor binding domain (RBD) located in the extracellular portion of the spike protein.
Alternative Names
SARS CoV 2 S glycoprotein; Sb68; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV; SARS-CoV2
UniProt
P0DTC2
Immunogen
This clone was originally isolated in a form of a synthetic nanobody (sybody) via a 'target swap' selection procedure against RBD-vYFP using ribosomal display and against RBD-Fc fusion during phage display rounds.
Application Notes
This antibody is recommended for detection of SARS CoV 2 or 2019-nCoV. Sb#68 is a strong binder and binds the 2019-nCoV ectodomain with an affinity of KD ≈ 37 nM (Walter et al., 2020). The initial characterization and binding activity of this antibody to SARS CoV2 spike protein was confirmed with ELISA (Walter et al., 2020). This antibody is a mild inhibitor for interaction between hACE2 and RBD of SARS CoV2. This clone binds to a non-overlapping epitope relative to S#15 (Ab02013) what can be used in develoepment of anti-Covid-19 therapuetics granting together increased avidity to the spike protein (Walter et al., 2020).
Antibody First Published
Walter et al. Sybodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv (2020)
Note on publication
Describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
