Cat. No.
MABL-255
Application
in vivo, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
15A10
From
Recombinant Antibody
Specificity
This antibody hydrolyzes the benzoyl ester of cocaine to form the nonpsychoactive metabolites benzoic acid and ecgonine methylester. Cocaine is a strong stimulant used clinically as a local analgesic and vasoconstrictor and illegally as a recreational drug.
Alternative Names
Benzoylmethylecgonine; coca; coke; crack; ChEBII: 27958
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with BSA conjugated transition state analogs of cocaine.
Application Notes
This catalytic antibody is capable of cocaine to its non psychoactive metabolites. The rate of acceleration of the catalytic antibody was reported to be (kcat/kuncat = 2.3 × 10^4). Competitive ELISA was used for the detection of binding of this antibody to the transition-state analogue of cocaine (Yang et al., 1996). In a model of cocaine overdose, this antibody protected rats from cocaine-induced seizures and sudden death in a dose-dependent fashion; a noncatalytic anticocaine antibody did not reduce toxicity. In a model of cocaine addiction, this antibody blocked completely the reinforcing effect of cocaine in rats (PMID: 9707620).
Antibody First Published
Landry et al. Anti-cocaine catalytic antibodies--a novel approach to the problem of addiction. J Addict Dis. 1997;16(3):1-17. PMID:9243335
Note on publication
Describes the generation of catalytic antibodies with hydrolytic activity against cocaine.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
