Cat. No.
MABL-3226
Application
WB, IF
Isotype
Engineer antibody
Species Reactivity
Vesicular stomatitis Indiana virus
Clone No.
1E9F9
From
Recombinant Antibody
Specificity
The IE9F9 (I14) monoclonal antibody reacts with folded VSV-G protein from the Indiana serotype, and has been successfully used in experiments with VSV-G TS045.
Alternative Names
VSV GP; vesicular stomatitis virus glycoprotein; glycoprotein; Glycoprotein; vcv-glycoprotein; vsv; vesicular stomatitis virus; Ie9F9; I14; G; G protein; G-protein; vesicular stomatitis Indiana virus; vesicular stomatitis Indiana virus Glycoprotein; VSV-Ind G; IE9F9
UniProt
Immunogen
The antibody was raised by immunizing mice with VSV.
Application Notes
IF: 1E9F9 anti-VCV-G antibody was used to detect cell-surface levels of VSV G-protein in studies on the rate of cellular transport in amyotrophic lateral sclerosis cellular model (Genevini et al., 2014). IF and WT: Yonemura et al. (2016) successfully used 1E9F9 antibody to perform immunofluorescence staining of HeLa cells which had been prevoiusly tranfected with VSVG-EYFP and detected VCVG protein on Western blots. IF: In folding experiments, 1E9F9 antibody stained VSVG in HEK293 cells transfected with VSVG-GFP (Tam et al., 2018).
Antibody First Published
Lefrancois and Lyles The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology. 1982 Aug;121(1):157-67. PMID:18638751
Note on publication
The article describes the method of generation of various antibodies against surface glycoprotein of two serotypes of the VSV: Indiana and New Jersey. Among them there is the IE9F9 (I14) antibody. The paper further characterises those antbodies and analyses their epitopes in relation to one another.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
