Cat. No.
MABL-3242
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
White spot syndrome virus
Clone No.
A1
From
Recombinant Antibody
Specificity
The antibody is specific for WSSV. The antibody binds to native WSSV, and not to the denatured WSSV.
Alternative Names
White spot syndrome virus
UniProt
Immunogen
The original antibody was generated by immunizing mice with WSSV followed by antibody library construction and phage display-based selection.
Application Notes
The binding specificity of the scFv antibody was determined by ELISA (Dai et al., 2003; PMID: 12969566 and Yuan et al., 2006; PMID: 17140131). The antibody had an affinity constant (Kaff) of 2.02 x 10^9 M-1. Dot blot assays showed that the antibody could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV (Dai et al., 2003; PMID: 12969566).
Antibody First Published
Dai et al. Construction and characterization of a novel recombinant single- chain variable fragment antibody against White Spot Syndrome Virus from shrimp J Immunol Methods. 2003 Aug;279(1-2):267-75. PMID:12969566
Note on publication
The original paper describes the generation and characterization of the antibody by phage display method.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
