Cat. No.
MABL-3246
Application
in vivo, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
MARX10
From
Recombinant Antibody
Specificity
This antibody binds mouse XCR1, which is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It transduces a signal by increasing the intracellular calcium ions level. This antibody is also reported to cross-reacts with rat CXR1.
Alternative Names
mXCR1; Ccxcr1; Chemokine XC receptor 1; Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1; MARX1O
UniProt
Q9R0M1
Immunogen
The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 × 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat- inactivated B. pertussis.
Application Notes
This antibody was used to stain cryostat sections (12 μm) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20% of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012; PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1+ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay; MARX10-OVA was found to be highly effective in inducing CD8+ T cell cytotoxicity in vivo (Hartung et al., 2015; PMID: 25520399). This antibody has been added to the Antibody Registry (RRID: AB_3668949).
Antibody First Published
Bachem et al. Expression of XCR1 Characterizes the Batf3-Dependent Lineage of Dendritic Cells Capable of Antigen Cross-Presentation. Front Immunol. 2012 Jul 18;3:214. PMID:22826713
Note on publication
XCR1 expression determines Batf3-dependent lineage of dendritic cells with a special capacity to cross-present antigen. The paper studies the expression of XCR1 on CD8+ dendritic cells using antibody MARX10 and its role in antigen presentation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
