Cat. No.
MABL-3263
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Fusarium species
Clone No.
QY1.5
From
Recombinant Antibody
Specificity
This antibody is specific for zearalenone and show some cross reactivity with hydroxylated zearalenone analogs. It is a mycotoxin produced mainly by fungi belonging to the genus Fusarium in foods and feeds. Possess estrogenic activity in pigs, cattle and sheep, with low acute toxicity. When fed to animals, the compound causes hypoestrogenism with symptoms such as enlargement of the uterus and nipples, vulvar swelling, vaginal prolapse, and infertility
Alternative Names
RAL; F-2 toxin; F-2 mycotoxin; Mycotoxin F2; [6-(10-hydroxy-6-oxo-trans- 1-undecenyl)-bresorcylic acid lactone]; CAS NO: 17924-92-4; ChEBI:10106
UniProt
Immunogen
The original antibody was generated using phage display technology by panning an scFv library against zearalenone-ovalbumin conjugate. The scFv library was created by linking the heavy and light chain genes of antizearalenone hybridoma cell line (2G3-6E3-2E2) using DNA linker encoding peptide (Gly4Ser)3 and cloning into a phagemid (pCANTAB5E) to be expressed as a fusion protein on the surfaces of recombinant phages. The original hybridoma (2G3-6E3-2E2) was generated using zearalenone-BSA conjugate as an immunogen.
Application Notes
The binding specificity of this scFv antibody towards mycotoxin zearalenone was determined using ELISA. The scFv version of this antibody was also able to detect zearalenone in spiked corn extract in a competitive-indirect ELISA (PMID: 8979354).
Antibody First Published
Yuan et al. Molecular cloning, expression, and characterization of a functional single-chain Fv antibody to the mycotoxin zearalenone. Appl Environ Microbiol. 1997 Jan;63(1):263-9. PMID:8979354
Note on publication
Describes the generation of a functional scFv antibody having specificity for mycotoxin zearalenone using phage display technology.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
