Cat. No.
MABL-3271
Application
neutralizing, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Zika Virus
Clone No.
G9E
From
Recombinant Antibody
Specificity
This antibody binds to ZIKV and does not cross-react with DENV4, which was tested as a representative DENV serotype. Its epitope is distinct from the A9E anti-ZIKV antibody.
Alternative Names
Zika envelope protein DII; ZIKV; Zika virus EDII; EDII
UniProt
Immunogen
This antibody was generated using 6XL method (Kwakkenbos et al., 2010). Memory B cells were isolated from a subject previously infected with ZIKV. After identification of ZIKV-specific monoclonal cultures, genetic material of the variable domains was isolated and recombinant IgG1 lambda antibody forms were grafted with the sequences.
Application Notes
This antibody has very strong neutralizing properties against Asian and African lineages of ZIKV (Collins et al., 2019). Furthermore, this antibody was effective in preventing Zika infection when administered from one day before the infections in mice (Collins et al., 2019). Therefore, A9E is recommended in development of diagnostic assays which differentiate ZIKV from other flaviviruses (it does not crossreact with DENV) and in the development of therapeutics and vaccines against ZIKV.
Antibody First Published
Collins et al. Human antibody response to Zika targets type-specific quaternary structure epitopes. JCI Insight. 2019 Apr 18;4(8). Pii: 124588. doi: 10.1172/jci.insight.124588. eCollection 2019 Apr 18 PMID:30996133
Note on publication
This article describes the generation and characterization of the G9E antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
