Cat. No.
MABL-3323
Application
lysis, ELISA
Isotype
Engineer antibody
Species Reactivity
Cow, Rabbit, Mouse
Clone No.
B1H12
From
Recombinant Antibody
Specificity
This antibody is specific for α-gal epitopes (Galα1–3Galβ1–4GlcNAc-R) on glycoprotein and glycolipid molecules.
Alternative Names
alpha gal; Galα1–3Galβ1–4GlcNAc-R; alpha gal; gal
UniProt
Immunogen
This antibody was raised by immunising α1,3galactosyltransferase knock-out mice, which lack α-gal epitopes. Mice were immunised with rabbit red cell membranes, on which α-gal is the major carbohydrate epitope.
Application Notes
This antibody binds to glycoprotein and glycolipid molecules expressing α-gal epitopes, including α-gal-BSA, in ELISA analysis (Chen et al., 2005). It does not cross-react with any glycoproteins lacking this epitope. This antibody binds to the α-gal epitopes on the hybridoma cells producing the antibody, so can mediate complement mediated autolysis in the presence of rabbit serum (Chen et al., 2000).
Antibody First Published
Chen et al. Genes coding evolutionary novel anti-carbohydrate antibodies: studies on anti-Gal production in α1,3galactosyltransferase knock out mice Molecular Immunology, 2000, Vol.37(8), pp.455-466 PMID:11090880
Note on publication
Describes the original generation of this antibody, and its characterisation in ELISA analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
