Key features and details | |
Cat. No. | MABL-351 |
Name | Anti-Acrolein-modified protein mAbs |
Clone No. | AFD- 5F6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IHC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for acrolein-modified proteins. Protein modification by acrolein is largely attributed to the formation of acrolein-lysine and acrolein-histidine adducts that possess a carbonyl function. While the antibody has been raised against acrolein-modified KLH, it has been shown to recognize both acrolein-modified BSA and oxidized LDL. |
Alternative Name | Acrolein modified protein; ACR-P; ACRL |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with an acrolein-treated keyhole limpet hemocyanin (KLH). |
Application Notes | A direct ELISA examined the immunoreactivity of this antibody to aldehyde-treated proteins. It was scarcely inhibited by Na -acetyl-Nim-propanal-histidine and significantly inhibited by Na - acetyl-FDP-lysine, indicating that FDP-lysine (Nε -(3-formyl-3,4-dehydropiperidino)lysine) is an epitope. This antibody was also used for immunohistochemistry (IHC) detection of protein-bound acrolein in fatty streak lesions of arterial tissue (Uchida et al., 1998; PMID: 9560197). This antibody was used in flow cytometry (FC) to detect protein-bound acrolein in activated human T cells (Fonseca et al., 2001; PMID: 11342443). A novel pyridinium-type lysine adduct, Nϵ -(3-methyl-pyridinium)lysine (MP-lysine), was investigated and found to be a major epitope of this antibody (Furuhata et al., 2003; PMID: 14504272). This antibody was used for IHC to detect oxidative stress in the testis of rats (Iuchi et al., 2004; PMID: 14673659). This antibody was used for WB to detect acrolein modification of apoE3-NT (Tamamizu-Kato et al., 2007; PMID: 17580963). |
Antibody First Published | Uchida et al. Protein-bound acrolein: potential markers for oxidative stress. Proc Natl Acad Sci USA. 1998 Apr 28;95(9):4882-7. doi: 10.1073/pnas.95.9.4882 PMID:9560197 |
Note on publication | The original publication describes the development of the antibody, which recognizes protein-bound acrolein, a potential marker of oxidative stress and long-term protein damage inaging, atherosclerosis, and diabetes. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
