Key features and details | |
Cat. No. | MABL-3393 |
Name | Anti-IL-1B mAbs |
Clone No. | AFD-MAb201 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, inhibit, neutralize, WB |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human Interleukin-1 beta, which is a potent pro-inflammatory cytokine. This antibody is reported to show some cross reactivity with recombinant mouse IL-1b. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. It promotes Th17 differentiation of T-cells and synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. It also plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. |
Alternative Name | IL1B; IL-1β; 1L1F2; Interleukin-1 beta; IL-1 beta; Catabolin |
UniProt | P01584 |
Immunogen | The original antibody was generated by immunizing mice with E. coli derived recombinant human IL-1 beta /IL-1F2 (aa 117-269). |
Application Notes | This antibody can be used in the detection of human IL-1b using western blot (PMID: 19465481). This antibody was used for the immunocytochemical staining of nuclear IL-1b in human breast cancer samples (PMID: 21057224). This antibody has been reported to neutralize the human IL-1 beta induced stimulation of proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner. This antibody inhibits binding of IL-1RAcP to IL-1 bound IL-1R1. This antibody is also reported to be as potent as IL-1ra in inhibiting IL-1 induced IL-6 in human chondrocytes. (US20030026806). Humanized version of this antibody is available on request. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
