Cat. No.
MABL-3871
Application
Block, FC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RMP1-14
From
Recombinant Antibody
Specificity
This antibody binds to murine PD-1.
Alternative Names
CD279; PD1; PDCD1; RMPI-14; RMP1/14; Programmed Death Receptor 1; Programmed Death 1
UniProt
Immunogen
The original rat hybridoma was generated by immunizing Sprague Dawley rats with mouse PD-1-transfectked BHK cells. A P3U1 myeloma was used as the fusion partner.
Application Notes
Binding of the original rat IgG2a antibody to PD1 has been reported to prevent binding of both B7-H1-Ig (PD-L1-Ig) and B7-DC-Ig (PD-L2-Ig) fusion proteins to PD1.
Antibody First Published
Yamazaki et al. Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production. J Immunol. 2005 Aug 1;175(3):1586-92 PMID:16034097
Note on publication
Describes the generation and initial characterisation of the RMP1-14 hybridoma
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
