Cat. No.
MABL-3872
Application
Blocking, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
10F.9G2
From
Recombinant Antibody
Specificity
This antibody is specific for murine PD-L1.
Alternative Names
CD274; PDL1; PD L1; Programmed cell death 1 ligand 1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; 10F9G2; 10F 9G2
UniProt
Q9EP73
Immunogen
This antibody was raised by immunising Lewis rats with murine PD-L1 cDNA and murine PD- L1 CHO transfectants.
Application Notes
The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1 expression in frozen tissue sections of murine brain (Eppihimer et al, 2002) and murine hearts (Rodig et al, 2003). This antibody has been shown to block the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This antibody precipitates diabetes in NOD mice and in adoptive transfer models of CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011).
Antibody First Published
Eppihimer et al. Expression and Regulation of the PD-L1 Immunoinhibitory Molecule on Microvascular Endothelial Cells Microcirculation. 2002 Apr;9(2):133-45. PMID:11932780
Note on publication
Describes the original generation of this antibody, and its use in ELISA, flow cytometry and immunohistochemistry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
