Cat. No.
MABL-3885
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
196-14
From
Recombinant Antibody
Specificity
The antibody binds to CA125 with a Kd of 0.83 nM reported for the original antibody.
Alternative Names
MUC16; Cancer antigen 125; carcinoma antigen 125; carbohydrate antigen 125; mucin 16
UniProt
Q8WXI7
Immunogen
The original mouse antibody OC125 was generated by immunizing BLAB/c with an epithelial cell line OVCA433 established from a patient (E.M.) with serous papillary cystadenocarcinoma. This antibody is a recombinant version derived from the murine parent antibody OC125.
Application Notes
This recombinant antibody is derived from the parent mouse IgG1 antibody OC125. The original mouse IgG1 version of this antibody was found to bind 6 different epithelial ovarian carcinoma cell lines. The binding characterization of the original mouse IgG1 version of this antibody was done using flow cytometry and indirect immunofluorescence staining. The original antibody was used for the immunofluorescence staining of ovarian papillary serous adenocarcinoma(PMID: 7028788). The original mouse IgG1 antibody, OC125, was used in the radioimmunoprecipitation of antigens from [3H]GlcN-labeled OVCAR-3 cells. Inhibition and indirect ELISA was used to test the effect of various enzymic and chemical treatments on binding of the original mouse IgG1 version of this antibody towards CA125. This antibody was also used in the identification of CA125 antigen in a western blot assay (PMID: 9180155). The original mouse IgG1 antibody was used in a immunohistochemical study where 22 different anti-CA125 antibodies were tested for their properties in staining various tissues from normal proliferative endometrium (formalin-fixed paraffin-embedded) material and clear-cell adenocarcinoma of the ovary (formalin-fixed paraffin-embedded and frozen material) (PMID: 8938947).
Antibody First Published
Bast et al. Reactivity of a monoclonal antibody with human ovarian carcinoma. J Clin Invest. 1981 Nov;68(5):1331-7. PMID:7028788
Note on publication
Describes the generation of an antibody that reacts with human epithelial ovarian carcinoma.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
