Cat. No.
MABL-3900
Application
FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B4
From
Recombinant Antibody
Specificity
This antibody reacts with the B4 antigen present on the peripheral blood cells and lymphoid organs, on cell lines derived from normal and malignant B cells, and on tumor cells isolated from patients with B cell-derived neoplasms
Alternative Names
B4 antigen; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12
UniProt
P15391
Immunogen
The original antibody was generated by immunizing mice with human YT lymphoma cells.
Application Notes
This antibody was used to study the expression of CD30 and other cell surface markers on YT lymphoma cells using flow cytometry. The binding of this antibody to CD30 induces inhibition of cytotoxicity of YT cells for Raji cells. CD30 stimulation by AC10 down-regulates CD28 expression on YT by >80%. C10 treatment of YT cells additionally downregulates the expression CD45 and up-regulates IL-2R p55 (PMID: 8245437). This antibody was used for the generation of an antibody-drug conjugate called Adcetris (Brentuximab vedotin). The chimeric version of the antibody cAC10 was conjugated to the cytotoxic agent monomethyl auristatin E (MMAE) via a valine-citrulline peptide linker to create the antibody-drug conjugate cAC10-val-cit-MMAE. The release of cytotoxic tubulin modifier MMAE into the cytosol induced G2/M-phase growth arrest and cell death through the induction of apoptosis. This ADC was approved by the FDA for the treatment of Hodgkin lymphoma and systemic anaplastic large cell lymphoma (PMID: 12714494).
Antibody First Published
Bowen et al. Functional effects of CD30 on a large granular lymphoma cell line, YT. Inhibition of cytotoxicity, regulation of CD28 and IL-2R, and induction of homotypic aggregation. J Immunol. 1993 Dec 1;151(11):5896-906. PMID:8245437
Note on publication
This paper describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
