Cat. No.
MABL-3436
Application
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
MCS2
From
Recombinant Antibody
Specificity
This antibody preferentially recognizes and binds the maltose-bound form of MBP and acts as positive allosteric effectors by significantly increasing the affinity for maltose.
Alternative Names
malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein
UniProt
P0AEX9
Immunogen
This conformation specific synthetic antibody was generated by utilizing a phage display library wherein the the Fab fragments is fused to the minor coat protein pIII of the M13 bacteriophage. To generate sABs that preferentially bind to the closed form of MBP (closed-specific sABs), the selection was carried out in the presence of 10 μM maltose. After fours rounds of selection the isolated colonies were subjected to competitive phage ELISA to provide an estimate of affinity towards MBP and specificity for closed form.
Application Notes
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
