Cat. No.
MABL-3437
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
MBP-7128
From
Recombinant Antibody
Specificity
This antibody recognizes and binds maltose binding protein of E. coli.
Alternative Names
malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein
UniProt
P0AEX9
Immunogen
The original antibody was generated as a diabody by fusing two different Fv domains by short peptide linkers.
Application Notes
Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. Maltose- binding protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure. In addition to protein-isolation applications, MBP provides a high degree of translation and facilitates the proper folding and solubility of the target protein (PMID: 20978971).
Antibody First Published
Kim et al. Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface. Sci Rep. (2016); 6; 34515. PMID:27682821
Note on publication
Describes the crystal structure of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
