Cat. No.
MABL-3450
Application
Depletion, functional assays, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
PC-61.5.3
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody.
Alternative Names
IL2-RA; IL-2 receptor subunit alpha;L-2R alpha chain; IL-2R subunit alpha; Interleukin-2 receptor subunit alpha; p55; p55 chain; PC61; PC6153
UniProt
P01590
Immunogen
This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.
Application Notes
This antibody has been used in numerous FACS analyses, for instance, to demonstrate that glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N- glycosylation (Araujo et al, 2017), to suggest that IL-2C could be a potential therapeutic method to alleviate excessive inflammation and maintain blood vessel stability after traumatic brain injury (Gao et al, 2017), and to evaluate how alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells (Meininger e al, 2016). This antibody has also been used in immunofluorescence as part of the DNA Aβ42 immunization studies in mice (Lambracht-Washington et al, 2015), in ELISA assays to investigate the dynamics of T cell receptor (TCR)-dependent signaling networks (Brockmeyer et al, 2011), and in Western Blot to determine the effects of increased p300 expression on glucocorticoid receptor (GR)-T-cell-receptor (TCR) crosstalk between thymocytes (Yu et at, 2002).
Antibody First Published
Trowbridge et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J Cell Physiol. 1982 Sep;112(3):403-10. PMID:6290505
Note on publication
Describe the original generation of this antibody and its subsequent characterisation by FACS analysis and in vitro biochemical assays.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
