Cat. No.
MABL-3451
Application
Blocking, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
1B4
From
Recombinant Antibody
Specificity
This antibody is specific for murine TIGIT.
Alternative Names
VSTM3; T cell Ig and ITIM domain; T cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain-containing protein 3; WUCAM
UniProt
P86176
Immunogen
This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
Application Notes
The binding specificity of this antibody for TIGIT has been confirmed in ELISA analysis and by flow cytometry using P815 cells transfected with mouse TIGIT and activated mouse splenocytes (Dixon et al, 2018). This antibody also specifically stains activated primary wild-type T cells (Dixon et al, 2018). This antibody fully blocks CD155 binding to TIGIT, and acts as a TIGIT blocking antibody in vivo. Administration of this antibody increases experimental autoimmune encephalomyelitis (EAE) severity, and retards the growth of established MC38 colon carcinoma and GL261 glioblastoma (Dixon et al, 2018). This antibody synergises with anti-PD-1 blocking antibodies to achieve complete tumour regression in colon carcinoma models (Dixon et al, 2018). The original mouse IgG1 antibody does not deplete TIGIT-expressing CD4+ or CD8+ T cells in vivo, nor affect T cell proliferation in vitro (Dixon et al, 2018).
Antibody First Published
Dixon et al. Functional Anti-TIGIT Antibodies Regulate Development of Autoimmunity and Antitumor Immunity J Immunol. 2018 Apr 15;200(8):3000-3007 PMID:29500245
Note on publication
Describes the original generation of this antibody, and the establishment of its TIGIT- blocking function.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
