Cat. No.
MABL-3452
Application
Blocking, IP, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
C9B7W
From
Recombinant Antibody
Specificity
This antibody is specific for an epitope in the D2 domain of murine LAG-3.
Alternative Names
CD223; LAG3; Lymphocyte activation gene 3 protein
UniProt
Q61790
Immunogen
This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
Application Notes
This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild‐type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen‐induced IL‐2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).
Antibody First Published
Workman et al. Phenotypic analysis of the murine CD4‐related glycoprotein, CD223 (LAG‐3) Eur J Immunol. 2002 Aug;32(8):2255-63 PMID:12209638
Note on publication
Describes the original generation of this antibody, and its characterisation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
