Cat. No.
MABL-3453
Application
Blocking, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
6A6
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD96, and recognises an epitope in the first Ig domain.
Alternative Names
TACTILE; T-cell surface protein tactile; T cell-activated increased late expression protein
UniProt
Q3U0X8
Immunogen
This antibody was raised by immunising rats with murine CD96.
Application Notes
This antibody blocks murine CD96-CD155 interaction, and has been shown to bind to the first Ig domain of CD96 and compete with CD155 binding (Roman Aguilera et al, 2018). This antibody displays significant anti-metastatic activity in four experimental lung metastases models and one spontaneous tumour metastasis model (Roman Aguilera et al, 2018), with a potency greater than the anti- CD96 monoclonal antibodies 3.3 and 8B10. The binding specificity of this antibody has been determined in flow cytometric analysis of HEK-293 cells transfected with a range of CD96 Ig domain chimeric constructs (Roman Aguilera et al, 2018).
Antibody First Published
Seth et al. The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochem Biophys Res Commun. 2007 Dec 28;364(4):959-65. PMID:17971293
Note on publication
Describes the original generation of this antibody, and its characterisation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
