Cat. No.
MABL-3455
Application
Blocking, functional assays, immunoblot, IP, WB, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
HMBT-6B2
From
Recombinant Antibody
Specificity
This antibody is specific for B and T lymphocyte attenuator (BTLA), which functions as a negative regulator of T cell activation and proliferation, and attenuates B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
Alternative Names
CD272; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B and T lymphocyte associated protein; B- and T-lymphocyte-associated protein; BTLA1; FLJ16065; MGC129743
UniProt
Q7TSA3
Immunogen
This antibody was raised by immunising Armenian hamsters with mouse BTLA:Fc fusion protein.
Application Notes
This antibody has been used in immunoblot analyses to confirm that B and T lymphocyte attenuator (BTLA) regulates T cell activation through interaction with herpesvirus entry mediator (HVEM) (Sedy et al, 2005), as well as in FACS and yeast display technology to demonstrate that BTLA exhibits structural and expression polymorphisms and is highly induced in anergic CD4+ T Cells (Hurchla et al, 2005). In a mouse conjunctivitis model, treatment with this antibody during the induction phase has been shown to decrease B-cell population, upregulate Th2 cytokine production and increase the conjunctival eosinophile numbers (Ishida et al, 2012). However, treatment with this antibody during the effector phase does not affect the development of experimental conjunctivitis (Ishida et al, 2012).
Antibody First Published
Waka Ishida et al. B and T lymphocyte attenuator regulates the development of antigen-induced experimental conjunctivitis. Graefes Arch Clin Exp Ophthalmol. 2012 Feb;250(2):289-95. PMID:21779950
Note on publication
Describe the use of this antibody, together with the anti-HVEM antibody, to investigate the roles that B and T lymphocyte attenuator (BTLA) and herpesvirus entry mediator (HVEM) play in the development of antigen-induced experimental conjunctivitis (EC).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
