Cat. No.
MABL-3456
Application
FACS, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
3A1
From
Recombinant Antibody
Specificity
This antibody is specific for murine TIM-4. TIM-4, unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen-presenting cells such as lymphoid and myeloid dendritic cells and macrophages. TIM-4 has been shown to interact with TIM-1, thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally, TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in the engulfment of apoptotic cells.
Alternative Names
TIMD4; SMUCKLER; SMUCKLER, TIM4; Spleen, mucin-containing, knockout of lymphotoxin protein; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIMD-4
UniProt
Q6U7R4
Immunogen
This antibody was generated by immunizing a female Lewis rat with Tim-4-Ig fusion protein.
Application Notes
The original version of this antibody (rat IgG1) specifically bound murine Tim-4 protein, as shown in ELISA and FC assays (Rodriguez-Manzanet et al., 2008; PMID: 18354194). This antibody can block apoptotic body (AB) recognition by CD11b+ peritoneal macrophages and partially inhibit AB uptake by peritoneal B-1 cells (Rodriguez-Manzanet et al., 2010; PMID: 20368430).
Antibody First Published
Rodriguez-Manzanet et al., TIM-4 expressed on APCs induces T cell expansion and survival J Immunol. 2008 Apr 1;180(7):4706-13. PMID:18354194
Note on publication
The original publication characterizes monoclonal antibodies generated to study how TIM-4 induces T-cell expansion and survival.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
