Cat. No.
MABL-3460
Application
Blocking, therapeutic, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
2C12
From
Recombinant Antibody
Specificity
This antibody is specific for murine TIM-3, a Th1-specific cell surface protein. TIM-3 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. This antibody binds to the BALB/c allele of TIM-3 while reactivity to the C57Bl/6 allele is significantly weaker.
Alternative Names
CD366; HAVcr-2; HAVCR2; Hepatitis A virus cellular receptor 2 homolog; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3; TIM3; 8B.2C12
UniProt
Q8VIM0
Immunogen
This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund’s adjuvant.
Application Notes
This antibody was used in FACS, immunohistochemistry, immunofluorescence and a murine experimental autoimmune encephalomyelitis model to study the effects of TIM-3 triggering in dendritic cells (Anderson et al., 2007; PMID: 18006747). Furthermore, this antibody has been used in multiple FACS analyses for diverse immuno-oncological applications, such as to delineate how TIM-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response (Frisancho-Kiss et al., 2006), to demonstrate the reversal of T cell exhaustion and restoration of anti-tumor immunity upon co-targeting Tim-3 and PD-1 pathways (Sakuishi et al., 2010; PMID: 20819927), and to examine the therapeutic potential of dendritic cell based vaccines against malignant glioma (Dey et al., 2015). This antibody has also been used in immunohistochemistry to aid the elucidation of the effects of Th2 immune deviation on corneal allograft survival and possible mechanisms of graft rejection (Beauregard et al., 2005).
Antibody First Published
Monney et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease. Nature. 2002 Jan 31;415(6871):536-41. doi: 10.1038/415536a. PMID:11823861
Note on publication
The original publication explores the role of Tim-3 in regulating macrophage activation and influencing the severity of Th1-dependent autoimmune diseases.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
