Cat. No.
MABL-3461
Application
Blocking, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
AFS98
From
Recombinant Antibody
Specificity
This antibody recognises mouse M-CSFR (CD115, CSF-1R) which is a membrane protein that influences tissue macrophage and osteoclast differentiation and proliferation.
Alternative Names
CSF-1R; Macrophage colony-stimulating factor 1 receptor; Csf1r; CD115; CSF-1 receptor; EC 2.7.10.1; CSF-1-R; M-CSF-R; Proto-oncogene c-Fms
UniProt
P09581
Immunogen
This antibody was raised against CD115.
Application Notes
AFS98 has been widely used in various flow cytometric analyses to label CD115 antigen. For instance, one group used it to detect monocytes in a mouse blood sample (Buschor et al., 2019). Similarly, Huo and colleagues (2017) utilised this antibody to identify monocytes in mouse blood in order to assess whether myelopoiesis was not a cause of the increase in the monocytes number. This antibody has been also successfully used to deplete macrophages (Ho et al., 2016). Finally, it was also utilised in the immunofluorescence staining of tissue sections prepared from 4% paraformaldehyde-fixed and decalcified bones (Dong et al., 2016).
Antibody First Published
Sudo et al. Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene. 1995 Dec 21;11(12):2469-76. PMID:8545103
Note on publication
This article describes the generation and characterisation of the AFS98 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
