Cat. No.
MABL-3468
Application
Depletion, in vivo, FC, IHC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
YTS169.4
From
Recombinant Antibody
Specificity
This antibody recognises the murine CD8 cell surface antigen expressed by a subset of T lymphocytes.
Alternative Names
Leu2; CD8; CD8A; CD8a molecule; CD8a antigen; Leu2 T-lymphocyte antigen; Lyt-2; Lyt2OKT8; p32T-cell antigen Leu2; T-cell antigen; T cell co-receptor; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; T8 T-cell antigen
UniProt
P01731
Immunogen
Murine CD8 (Lyt-2).
Application Notes
The antibody has been shown to show depleting activity in vivo. Mice have two alleles for CD8, Lyt2.1, and Lyt2.2, which are restricted to certain mouse strains. Lyt2.1, for example, is expressed in the mouse strains CBA, AKR, C3H, and DBA, whereas Lyt2.2 is expressed in the mouse strains BALB/c and C57BL/6 (B/6). YTS 169.4 was originally raised against CBA mouse thymocytes. However, there are publications in which it has been used in C57BL/6 and BALB/c mice, wherein it was used specifically as a pan-reactive Lyt2 reagent (Tavaré et al., 2014; PMID: 24390540).
Antibody First Published
Cobbold et al. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo Nature. 1984 Dec;312(5994):548-51. doi: 10.1038/312548a0 PMID:6150440
Note on publication
The original publication describes the generation of YTS 169.4 and demonstrates the efficacy of unmodified monoclonal antibodies for in vivo depletion of cells and their potential for selectively manipulating different aspects of the immune response.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
