Cat. No.
MABL-3469
Application
Depletion, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
1A8
From
Recombinant Antibody
Specificity
Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.
Alternative Names
LGR-1 ; y-6G; GR1; Lymphocyte antigen 6G
UniProt
P35461
Immunogen
LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
Application Notes
Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion
Antibody First Published
Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. Journal of Immunology PMID:8360469
Note on publication
Describes production of 1A8 and identification of Ly6G as its target.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
