Cat. No.
MABL-3473
Application
Blocking, functional assays, IP, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RG9-35
From
Recombinant Antibody
Specificity
This antibody is specific for murine galectin-9, a 40 kDa S-type lectin that is expressed by various cell types, including lymphocytes, thymocytes, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, epithelial cells, and endothelial cells. Galectin-9 binds to β-galactosides and can serve as a ligand for TIM-3 (CD366). The protein is implicated in both innate and adaptive immune responses, specifically, induction of cytokine secretion by macrophages, bactericidal functions, promotion of dendritic cell maturation, regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells.
Alternative Names
ecalectin; Gal-9; galectin 9; Galectin-9; HUAT; lectin, galactose binding, soluble 5; LGALS9A; tumor antigen HOM-HD-21; urate transporter/channel protein.
UniProt
O08573
Immunogen
This antibody was raised by immunising rats with recombinant mouse galectin-9.
Application Notes
When this antibody was first generated and characterised, it was shown to bind with galectin-9 (Gal-9) but not with either galectin-1 or galectin-3. Furthermore, it was shown to be capable of blocking Tim-3-Fc binding to Gal-9, and inhibiting Gal-9-induced Th1 cell death in vitro (Fukushima et al., 2008; PMID: 18087160). This antibody has been used in FACS analyses. For instance, to describe a novel mechanism where Tim-3 binds HMGB-1 on virus-specific CD8+ Treg cells, suppressing proliferation of CD8+ T effector cells during acute adenoviral infection (Dolina et al., 2014; PMID: 24677194); and to indicate a crucial role for Tim-3 in phagocytosis of apoptotic cells and cross-presentation (Nakayama et al., 2009; PMID: 19224762). In addition, this antibody has been used in vivo to study the roles of Gal-9 in the development of experimental allergic conjunctivitis in mice (Fukushima et al., 2008; PMID: 18087160) and to demonstrate that constitutive expression of Gal-9 plays an immunosuppressive role in corneal allografts (Shimmura-Tomita et al., 2013; PMID: 23667648).
Antibody First Published
Fukushima et al. Roles of galectin-9 in the development of experimental allergic conjunctivitis in mice. Int Arch Allergy Immunol. 2008;146(1):36-43. PMID:18087160
Note on publication
Describe the original generation of this antibody and its subsequent use in in vitro and in vivo functional assays to study the roles of galectin-9 in the development of experimental allergic conjunctivitis in mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
