Cat. No.
MABL-3472
Application
deplete, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RB6-8C5
From
Recombinant Antibody
Specificity
This antibody is specific for murine Ly6G, a a 21-25 kDa glycosylphosphatidylinositol (GPI)- linked protein expressed by the myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express Ly6G transiently during bone marrow development, while Ly6G expression in granulocytes and peripheral neutrophils directly correlates with the cell’s level of differentiation and maturation. This hallmark makes Ly6G a good marker for these particular cell populations. Ly6G has also been implicated in the development of antitumor responses.
Alternative Names
Gr-1; Gr1; Ly-6G; ly-6G.1; ly6C
UniProt
P35461
Immunogen
This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
Application Notes
This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to elucidate the origin and roles of macrophages in the post-myocardial infarction remodelling (Sager et al, 2016), to investigate the pathogenesis of primary biliary cirrhosis (Yang et al, 2016), to confirm the phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice (Ricard et al, 2016), and to highlight an important ageing-related defect in the mucosal immune system's ability to sample lumenal antigens (Kobayashi et al, 2013). This antibody has also been used in vivo, for example, to deplete Treg cells when delineating the paralleled developments of inflammation and immunosuppression in tumor microenvironment (Yang et al, 2010), or to deplete neutrophils when underpinning a causal role for neutrophils in severe asthma (Wilson et al, 2009).
Antibody First Published
Stephen Hurst et al. New IL-17 family members promote Th1 or Th2 responses in the lung: in vivo function of the novel cytokine IL-25. J Immunol. 2002 Jul 1;169(1): 443-53. PMID: 12077275
Note on publication
Describe the use of this antibody for FACS analysis and in vivo cell depletion to demonstrate the function of the novel cytokine IL-25 in the lung.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
