Cat. No.
MABL-3471
Application
IP, WB, Block, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
TY25
From
Recombinant Antibody
Specificity
This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1.
Alternative Names
CD273; B7-DC; PDL2; B7DC; programmed death ligand 2; CD273; Pdcd1lg2
UniProt
Q9WUL5
Immunogen
This antibody was raised by immunising SD rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected NRK.
Application Notes
This antibody has been used to detect murine PD-L2 by flow cytometry on stimulated dendritic cells as well as various mouse tumour cells. It also detects immunoprecipitates mouse PD-L2 and can be used to detect it by non-reducing Western Blot. TY25 inhibits PD-L2 binding to PD-1 transfected cells (Ansari et al. 2003, PMID: 12847137) and thereby blocks PD-L2: PD-1 signalling.
Antibody First Published
Yamazaki et al. Expression of programmed death 1 ligands by murine T cells and APC. J Immunol. 2002 Nov 15;169(10): 5538-45 PMID:12421930
Note on publication
Describes the generation and initial characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
