Cat. No.
MABL-3475
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
3F1
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD155.
Alternative Names
TAGE 3F1; Poliovirus receptor; cellular receptor for poliovirus; Pvr; Tumor-associated antigen 1; Taa1; Tage4; HVED
UniProt
Q8K094
Immunogen
This antibody was raised by immunising rats with recombinant murine CD155, consisting of the entire ectodomain expressed as a HIS-tagged protein.
Application Notes
The binding specificity of this antibody has been confirmed both in ELISA analysis using recombinant murine CD155 (mCD155), and in flow cytometric analysis using HEK293 cells transiently expressing mCD155 (Maier et al, 2007). This antibody has been used in flow cytometry to assess mCD155 expression on lymphocytes and dendritic cells (Maier et al, 2007; Seth et al, 2011). This antibody blocks the interaction of CD155 with its ligands CD96 and CD226 (Qiu et al, 2010). When administered to WT mice, antibody-induced disruption of the CD155-CD226 interaction has been shown to cause CD226 upregulation on CD4+ T cells and CD8+ T cells (Seth et al, 2011). Administration of this antibody has also been shown to stimulate the loss of mature, memory-like CD8+ T cells from the thymus (Seth et al, 2011; Georgiev et al, 2016).
Antibody First Published
Maier et al. The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens Eur J Immunol. 2007 Aug;37(8):2214-25. PMID:17621371
Note on publication
Describes the original generation of this antibody, as part of a panel of anti-CD155 antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
