Cat. No.
MABL-3476
Application
FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
10E 5
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD226.
Alternative Names
DNAM-1; PTA1; PTA-1; platelet and T cell activation antigen 1; TLISA1
UniProt
Q8K4F0
Immunogen
This antibody was raised by immunising Lewis and Lou/M rats with the murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice, before screening for reactivity with Th1 clones and a lack of reactivity to Th2/Th0 cell lines.
Application Notes
The specificity of this antibody for murine CD226 has been confirmed in flow cytometric analysis using 293 cells transfected with CD226 cDNA and untransfected 293 cells (Dardalhon et al, 2005). This antibody has been used in flow cytometry to identify the surface expression pattern of CD226, which is predominantly confined to T cells (Dardalhon et al, 2005; Yasuma et al, 2016), and to detect NK cell CD226 expression and apoptosis (Huang et al, 2011). Immunisation of mice with this antibody inhibits antigen-specific T cell expansion (Dardalhon et al, 2005). Additionally, treatment of experimental autoimmune encephalomyelitis (EAE) mice with this antibody delays the onset and reduces the severity of Th1 cell-mediated autoimmune disease (Dardalhon et al, 2005).
Antibody First Published
Dardalhon et al. CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. J Immunol. 2005 Aug 1;175(3):1558-65. PMID:16034094
Note on publication
Describes the original generation of this antibody, and its characterisation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
