Cat. No.
MABL-3477
Application
WV, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RA3-6B2
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD45R antigen which is expressed on B lymphocytes throughout their development from early pro-B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R mAb clone RA3-6B2 specifically recognizes the exon A–restricted isoform of mouse CD45.
Alternative Names
B220; CD45; CD45 antigen; Cluster designation 45R; L-CA; Leukocyte common antigen; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
UniProt
P06800
Immunogen
This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
Application Notes
This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to understand the role of PD-1 in maintaining islet- specific tolerance in type 1 diabetes (Martinov et al, 2016), to investigate how Staphylococcus aureus enterotoxins spread systemically and trigger inflammatory cascades (Svedova et al, 2016), to highlight the physiologic importance of cell-type specific balancing of AhR/AhRR expression in response to microbial, nutritional and other environmental stimuli (Brandstätter et al, 2016), and to examine the role of FBXW7 in suppression of cancer metastasis in a non-cell-autonomous manner (Yumimoto et al, 2015).
Antibody First Published
Stephen Hurst et al. New IL-17 family members promote Th1 or Th2 responses in the lung: in vivo function of the novel cytokine IL-25. J Immunol. 2002 Jul 1;169(1):443-53. PMID:12077275
Note on publication
Describe the use of this antibody for FACS analysis to demonstrate the function of the novel cytokine IL-25 in the lung.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
