Cat. No.
MABL-3482
Application
IP, WB, Block, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
mIAP301
From
Recombinant Antibody
Specificity
MIAP301 binds specifically to the extracellular domain of CD47 (exon 2 that encodes both the predicted signal peptide clevage site and the entire Ig domain). CD47 plays a role in cell adhesion by acting as an adhesion receptor for THBS1 on platelets and in (beta-3) integrin modulation. Functions as a SIRPA receptor - binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 also interacts with SIRPG which mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. Plays an important role in memory formation and synaptic plasticity in the hippocampus.
Alternative Names
IAP; Leukocyte surface antigen CD47; Integrin-associated protein; integrin associated protein; MIAP 301; MIAP-301
UniProt
Q61735
Immunogen
MIAP301 was prepared by immunizing rats with intact CD47 purified from mouse placenta.
Application Notes
MIAP301 can block murine CD47 interactions with SIRPα and functionally blocks CD47 in vivo in murine soft tissue ischemia and skin graft models (Soto-Pantoja et al. 2012). This antibody has also been used for FC (Olsson et al. Blood. 2005 May 1; 105(9): 3577–3582.). MIAP301 has also been shown to block KCl-evoked glutamate release from the hippocampus in mice, inhibiting glutamate release and thus impair memory retention (Chang et al. 2001).
Antibody First Published
Lindberg et al. Decreased Resistance to Bacterial Infection and Granulocyte Defects in lAP-Deficient Mice Science. 1996 Nov 1;274(5288):795-8. PMID: 8864123
Note on publication
Describes the use of MIAP301 in determining CD47 tissue distribution and to ensure no functional fragment of CD47 was expressed in CD47 -/- KO mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
