Cat. No.
MABL-3483
Application
Blocking, functional assays, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RM80
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD80, a ~55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to the activation of T and B cells, respectively.
Alternative Names
B7-1; Activation B7-1 antigen; B7; B7 protein; B7-1 protein; B7.1; Cd28l; CD28LG; CD28LG1; LAB7; Ly-53; Ly53; MIC17; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1
UniProt
Q00609
Immunogen
This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
Application Notes
This antibody has been used in multiple FACS experiments for various immunological applications, such as to highlight the robustness of the T cell priming process by activated Nlrc5-deficient dendritic cells (Rota et al, 2016), to demonstrate that allogeneic IgG combined with dendritic cell stimuli could induce anti-tumor T cell immunity (Carmi et al, 2015), to confirm the role of aquaporin-9-expressing neutrophils for the development of contact hypersensitivity (Moniaga et al, 2015), and to support the therapeutic potential of targeting B-cell Syk signaling in chronic graft-versus-host disease (Flynn et al, 2015). This antibody has also been used in Western Blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al, 2015), as well as in in vitro blocking experiments to confirm the role of CD28 signals for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets (Ribot et al, 2012).
Antibody First Published
Atsuo Nakajima et al. Preferential dependence of autoantibody production in murine lupus on CD86 costimulatory molecule. Eur J Immunol. 1995 Nov;25(11):3060-9. PMID: 7489744
Note on publication
Describe the use of this antibody, together with the anti-CD86 antibody, to confirm the preferential dependence of murine Ab-mediated autoimmunity on the CD86 costimulatory molecule.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
