Cat. No.
MABL-3485
Application
Blocking, IHC, WB, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
29F.1A12
From
Recombinant Antibody
Specificity
This antibody recognizes an epitope on mouse PD-1.
Alternative Names
CD279; Programmed cell death protein 1; Protein PD-1; mPD-1
UniProt
Q02242
Immunogen
The antibody was raised by immunizing rats with plasmid DNA containing PD-1.
Application Notes
This antibody was used for detection of PD-1 in CHO cells using flow cytometry. The ability of the antibody to block PD-1 interaction with PD-L1 or PD-L2 on CHO cells in an in vitro blocking assay was tested; the clone blocked PD-L1 Ig and PD-L2 Ig binding at high concentration, but only blocked 35% of PD-L1 Ig or PD-L2 Ig binding at 10 ug/ml. This antibody was also used for immunohistochemical studies of various mouse tissues, such as heart (Liang et al, 2003; pmid: 14515254). In vivo anti-tumor effect in terms of level of tumor growth reduction was studied, a significant tumor growth reduction was observed in the tumors from mice treated with the antibody (Tobias et al, 2020; pmid: 32528470). The antibody was used for the detection of PD-1 in EL4 cells using flow cytometry. The antibody was used for cross-blocking with the unconjugated version of the same clones and it was reported to prevent PD-1 from interacting with PD-L1 (Polesso et al, 2021; pmid: 33684223). The antibody was used as a positive control for CT26 tumor model, a murine model of colon cancer (Kaumaya ey al.; pmid: 33117602).
Antibody First Published
Liang et al. Regulation of PD-1, PD-L1, and PD-L2 expressionduring normal and autoimmune response Eur. J. Immunol. 2003. 33: 2706–2716 PMID:14515254
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
