Cat. No.
MABL-3486
Application
Block, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
mam82
From
Recombinant Antibody
Specificity
This antibody specifically binds PD-1H.
Alternative Names
VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set immunoregulatory receptor
UniProt
Q9D659
Immunogen
The antibody was generated by immunising a PD-1H-KO mouse with mouse PD-1H Ig fusion protein and adjuvant.
Application Notes
This antibody has been characterised by ELISA and by staining of PD-1H-transfected P815 mastocytoma. No background staining of WT or PD-1H-KO T cells was observed. This antibody inhibits WT OT-II T cell proliferation at high OVA peptide concentrations (Flies D et al, 2014).
Antibody First Published
Flies D et al. Coinhibitory receptor PD-1H preferentially suppresses CD4+ T cell–mediated immunity J Clin Invest. 2014 May 1; 124(5): 1966–1975. PMID:24743150
Note on publication
Describes the generation of the antibody and its use to validate a coinhibitory function of PD-1H.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
