Cat. No.
MABL-3487
Application
Block, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
7E.17G9
From
Recombinant Antibody
Specificity
This anitbody binds to mouse ICOS (CD278).
Alternative Names
CD278; AILIM; CVID1; inducible T-cell co-stimulator; inducible T-cell costimulator; 7E.17G9.G1.G7
UniProt
Immunogen
Rats were immunized i.m. three times with 500 mg murine ICOS cDNA in the pAXEF mammalian expression vector and boosted twice with 200 mg murine ICOS hexahistidine fusion protein.
Application Notes
This antibody can be used to detect mouse ICOS by flow-cytometry. Maazi et al (2015, PMID: 25769613) showed that 7E.17G9 can inhibit ICOS interaction with its ligand ICOSL.
Antibody First Published
McAdam et al. Mouse Inducible Costimulatory Molecule (ICOS) Expression Is Enhanced by CD28 Costimulation and Regulates Differentiation of CD4+ T Cells J Immunol. 2000 Nov 1;165(9):5035-40. PMID:11046032
Note on publication
Describes the generation of this antibody and its use in detecting ICOS by flow- cytometry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
