Cat. No.
MABL-3489
Application
agonist, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
OX86
From
Recombinant Antibody
Specificity
This antibody recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. It is a member of TNFR-superfamily of receptors and acts as a costimulatory molecule involved in long-term T-cell immunity.
Alternative Names
OX40; OX-40; Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; OX-86
UniProt
P47741
Immunogen
This antibody was raised against the recombinant mouse OX40 protein.
Application Notes
OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc- dependent manner and has been widely used in studies investigating the role of OX40 in tumor inhibition (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement in the antitumor activity of this antibody; the study demonstrated that a stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et al., 2016). In another study two versions of the OX86 antibody (with a fully functional Fc fragment and with an impaired Fc) and showed that Fc-dependent activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi et al., 2018). FC: This antibody was also used in flow cytometry to stain mouse CD4 and CD8 single-positive cells (al-Shamkhani et al., 1996). FC: Blanquiceth et al. (2016) used this antibody in flow cytometry as well to analyse cells coming from mediastinal lymph nodes and lung tissue.
Antibody First Published
al-Shamkhani A, Birkeland ML, Puklavec M, Brown MH, James W, Barclay AN. OX40 is differentially expressed on activated rat and mouse T cells and is the sole receptor for the OX40 ligand. Eur J Immunol. 1996 Aug;26(8)：1695-9. PMID:8765008
Note on publication
Describes the generation of the antibody, its use in flow cytometry (staining mouse CD4 and CD8 single-positive cells).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
