Cat. No.
MABL-3490
Application
FC, blocking, neutralizing
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
CX-5
From
Recombinant Antibody
Specificity
This antibody recognizes mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1 thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.
Alternative Names
CD314; KLRK1; NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; CX5
UniProt
O54709
Immunogen
The original rat IgG1 version of this antibody was raised by immunizing rats against mouse KLRK1.
Application Notes
It has been shown that CX5 is a non-depleting antibody, which blocks binding of NKG2D to its ligands and mediates internalization of the receptor. This antibody has been widely used to block NKG2D (Wang et al., 2018; pmid: 29447423; Yang et al., 2017; pmid: 28455530) as well as to detect NKG2D-posotive cells via flow cytometry (Hosomi et al., 2017; pmid: 28747426).
Antibody First Published
Ogasawara et al. Impairment of NK cell function by NKG2D modulation in NOD mice Immunity. 2003 Jan;18(1):41-51. doi: 10.1016/s1074-7613(02)00505-8. PMID:12530974
Note on publication
Describes the characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
