Cat. No.
MABL-3491
Application
agonist, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
4D4
From
Recombinant Antibody
Specificity
4D4 specifically recognises TIGIT.
Alternative Names
VSIG9; T Cell Immunoreceptor With Ig And ITIM Domains; V-Set And Immunoglobulin Domain Containing Protein 9; V-Set And Transmembrane Domain Containing Protein 3; VSTM3; T Cell Immunoreceptor With Ig And ITIM Domains; Washington University Cell Adhesion Molecule; WUCAM
UniProt
P86176
Immunogen
Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
Application Notes
4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti- CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue (Liu 2016).
Antibody First Published
Joller et al. Cutting edge: TIGIT has T cell-intrinsic inhibitory functions. J Immunol. 2011 Feb 1;186(3):1338-42 PMID:21199897
Note on publication
Describes the generation of anti-TIGIT monoclonal antibodies and their use to analyse the function of TIGIT in cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
