Cat. No.
MABL-3492
Application
Depletion, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
2E 4
From
Recombinant Antibody
Specificity
2E4 recognizes mouse CD25. This epitope is distinct from the IL-2 binding site and does not block binding of IL-2 to CD25. This antibody binds the same or overlapping epitope as 7D4 (Ab02216) but different than Ab01107. Murine CD25 is a 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor.
Alternative Names
IL-2-RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2-RA; p55
UniProt
P01590
Immunogen
The original version of this antibody was raised to recognize mouse CD25.
Application Notes
2E4 is a non-IL-2 blocking anti-CD25 antibody. The variable fragment (Fv) of this antibody conjugated to a 38-kDa portion of Pseudomonas exotoxin A exhibited exceptional ability to induce tumor rejection and lasting antitumor immunitiy in mice making it a promising canditate in the immunotherapy research and developemnt (Onda et al., 2019; pmid: 30760587). This 2E4 Fv-based CD25- specific recombinant immunotoxin (RIT) was injected into three different mouse tumor models and brought about complete regressions of the majority of them leadinig, in many cases, to immunity against tumors' reinjections and cross-tumor type immunity (Onda et al., 2019; pmid: 30760587). Finally, it was demonstrated that this immunotoxin (RIT) caused significant depletion of regulatory T cells (Tregs) in the tumor environment but, importantly, not in the spleen; at the same time, the number of effector, cytotoxic CD8 cells in the tumor increased what would suggest the possible antitumour mechanism of this immunotoxin (Onda et al., 2019; pmid: 30760587). Additionally, 2E4 can be used in flow cytometric analyses; for instance, it has been used to perform phenotyping of lymphocytes as a part of a wider panel of antibodies (Alexander et al., 2008; pmid: 18824594).
Antibody First Published
Ortega et al. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells J Immunol. 1984 Oct;133(4):1970-5. PMID:6206144
Note on publication
Describes the characterization of the antibody 2E4.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
