Cat. No.
MABL-3547
Application
crystallization, IP, NTRL, IF
Isotype
Engineer antibody
Species Reactivity
Ebola (Zaire)
Clone No.
KZ52
From
Recombinant Antibody
Specificity
This antibody binds to the Ebola virus surface glycoprotein in its trimeric, pre-fusion conformation (GP1+GP2). KZ52 recognizes a protein epitope at the chalice base where it clamps several regions of the pre-fusion GP2 to the amino terminus of GP1.
Alternative Names
trimeric prefusion Ebola virus glycoprotein; EBOV GP
UniProt
Immunogen
This antibody was derived from a human survivor of the 1995 Kikwit outbreak of Ebola.
Application Notes
This antibody neutralizes Zaire ebolavirus in vitro and offers protection from lethal EBOV challenge in rodent models, but has minimal effects on viral pathogenicity in non-human primates. KZ52 likely neutralizes by preventing rearrangement of the GP2 HR1A/HR1B segments and blocking host membrane insertion of the internal fusion loop. Alternatively, IgG KZ52 may sterically hinder access to the RBS or to a separate binding site of another cellular factor, especially if multiple attachment events are required for entry (Lee et al. 2009). This antibody will detect both live and fixed virus by IF (Maruyama 1999, PMID: 10364354) . It has also been used as a positive control in the development of anti-Ebola polyclonal goat antibodies (Boisen et al. 2015).
Antibody First Published
Maruyama et al. Ebola virus can be effectively neutralized by antibody produced in natural human infection. J Virol. 1999 Jul; 73(7): 6024–6030. PMID:18615077
Note on publication
Describes the generation and functional charactrization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
